- (1339-1381) Married to King Enrique II of Castile (1350), mother of King Juan I.
- Daughter of Juan Manuel, Lord of Villena, and his second wife Blanca Núñez de Lara.
- Step-sister of Constanza, wife of Pedro, King of Portugal, and mother of the future King Fernando.
- Juana and Constanza were daughters of Don Juan Manuel but from two different marriages..
Johana